铜绿微囊藻细胞内外有机物特性及氯消毒副产物

Characteristics of Intracellular and Extracellular Organic Components of Microcystic Aeruginosa and Their Byproducts Formation Potential During Chlorination

摘要:

分析比较了铜绿微囊藻细胞内外溶解性有机碳(DOC)、溶解性有机氮(DON)、藻毒素、嗅味物质Beta环柠檬醛等有机物质量浓度以及相对分子质量分布和亲疏水特性特征.结果表明，不同生长时期细胞内有机物含量较细胞外高3倍以上 (以DOC计算).藻细胞内有机物相对分子质量分布区间主要分成3个部分：小于1 kD(叶绿素,胡萝卜素,藻毒素,嗅味物质等)、40～800 kD(藻蓝蛋白)、大于800 kD(核糖体,蓝藻淀粉等);细胞外有机物分布主要集中在1～200 kD之间(约占90%)，小分子和大分子物质较少.此外，亲疏水性分析结果表明，细胞内外主要有机物均为中性亲水物质，质量分数分别为75.9%和52.7%.消毒副产物研究表明，细胞内外有机物三氯甲烷生成潜能分别为18.24 μg和26.72 μg·mg-1DOC;细胞内外总氯乙酸总生成潜能分别为66.98和58.56 μg·mg-1DOC.此外，细胞内外有机氮/有机碳较普通原水高3倍以上，ρ(DON)/ρ(DOC)=0.23.可能造成以有机氮为前体物的含氮消毒副产物的大量生成.同时，细胞内还大量存在细胞外不存在的致嗅物质Beta环柠檬醛等,藻毒素含量较细胞外也高数倍.

Abstract:

Characteristics of the intracellular and extracellular organic components (desolved organic carbon (DOC),desolved organic nitrogenous (DON),the algae toxin,βcyclocitral,molecular weight distribution and hydrophilichydrophobic property) have been investigated.The result indicates that the amount of intracellular organic material is 3~10 times higher than that of the extracellular materia.And the intracellular organic matter mainly has 3 parts:less than 1 kD (chlorophyll,renieratene,algae toxin,smell material and so on),40~800 kD (phycocyanin),more than 800 kD (ribosome,starch and so on).The molecular weight of extracellular organic matter mainly concentrates within 1~200 kD (approximately 90%).In addition,the main intracellular and extracellular organic components are both the neutral hydrophilic substances,the percentage of which are 83% and 57%,respectively;The chloroform,chloroacetic formation potential of the intracellular and extracellular organic components are 10.4 μg·mg-1,15.5 μg·mg-1 and 57.10 μg·mg-1and 49.94 μg·mg-1.Moreover,the proportion of DON and TOC is 1/7,3 times higher than common water source,which may increase the formation of chlorination byproduct nitrosamine.