溶菌酶脂质体的制备及其对生物膜的剥离作用

Lysozymeliposome Synthesis and Its Capability in Biofilm Removal

摘要:

采用逆向蒸发法制备了稳定的溶菌酶脂质体.在不锈钢表面培养出稳定生物膜后,分别利用溶菌酶和溶菌酶脂质体对其进行剥离.运用Zeta电位仪、扫描电子显微镜(SEM)和红外光谱(FTIR)对脂质体和生物膜进行表征.结果表明制备的脂质体平均粒径为80~100 nm,包封率为82.4%.相同浓度下溶菌酶及其脂质体对混合菌种形成的生物膜剥离效率分别达到62.4%和86.5%.溶菌酶脂质体在24 h内对生物膜和水体中微生物去除率分别达到89.6%和99.6%.因此,溶菌酶脂质体能够有效控制不锈钢表面生物膜污染风险.

Abstract:

Stable lysozymeliposomes were synthesized via reversephase evaporation.Lysozyme and its liposomes were separately used to remove the stable biofilms cultivated on stainless steel surface.The liposomes and biofilms were characterized by Zeta potential analyzer,transmission electron microscope(TEM),scanning electron microscope(SEM)and Fourier transform infrared(FTIR)spectrometer.The experimental results show that the liposomes are of the average diameter of 80~100 nm and an average entrapment efficiency of 82.4%.Under the same concentration condition,free lysozyme and lysozymeliposomes can effectively remove total biomass of the biofilm,which forms with a mixed bacterial consortium,up to 62.4 % and 86.5 %,respectively.Lysozymeliposome reduces bacterial in biofilm and solutions by 89.6% and 99.6%,respectively,in 24 hours.Therefore,lysozymeliposome can effectively control the biofilm pollution on the stainless steel surface.