ASBR中加载活性炭对葡萄糖厌氧发酵产氢的影响

Influence of activated carbon amended ASBR on anaerobic fermentative bio-hydgrogen production from glucose

摘要:

摘要： 在初始pH 6.0、温度60℃、不同HRT（48h、24h、16h、12h）条件下研究了粗、细活性炭载体的添加对ASBR利用葡萄糖厌氧发酵产氢的影响。结果表明添加活性炭载体能使ASBR系统运行更加稳定（出水pH和氢气产量波动较小），提高氢气产率和产氢速率。HRT=48h、24h、16h、12h时细活性炭生物载体的添加使得ASBR反应器氢气产率分别提高65%、63%、54%、56%。HRT=12h时添加细活性炭的ASBR产氢速率达到最大7.09±0.31 L-H2/L/d，相应的氢气产率为1.42±0.03 mol-H2/mol-glucoseadded。主要代谢产物为乙醇、乙酸、丙酸和正丁酸，其中乙酸和正丁酸占出水溶解性代谢产物的比例分别高达30%~34%和 46~66%，是典型的丁酸型发酵，加载活性炭可以提高ASBR反应器出水溶解性代谢产物浓度。

Abstract:

Abstract：Effect of activated carbon amended ASBR on fermentative bio-hydgrogen production from glucose was evaluated at HRTs ranging from 48h to 12h with initial pH of 6.0 and system temperature of 60℃. Experimental results showed that the performance of activated carbon amended ASBRs were more stable than that of ASBRs without activated carbon addition regarding on hydrogen production and pH. Higher hydrogen yield(HY) and hydrogen production rate(HPR) were observed in the activated carbon amended ASBRs, with 65%、63%、54%、56% enhancement of hydrogen yield in smaller size activated carbon amended reactor under the tested HRTs ranges, and the maximum HPR of 7.09±0.31 L-H2/L/d and HY of 1.42±0.03 mol-H2/mol-glucose was obtained at HRT of 12h. The major soluble products form hydrogen fermentation were n-butyric acid and acetic acid, accounting for 46-66% and 30%-34% of total soluble metabolic products(SMP), respectively, indicating that the dominant H2 producers in the mixed culture belonged to acidogenic bacteria that underwent butyrate-type fermentation. In addition, higher concentration of VFA generation was observed in the activated carbon amended ASBRs.