胞壁结合型生物破乳菌Alcaligenes sp. S-XJ-1氮源利用特性

Characteristics of Nitrogen Sourceutilization for Cell-bound Biodemulsifying Strain of Alcaligenes sp. S-XJ-1

摘要:

以六种不同氨基酸及硝酸铵做氮源培养胞壁结合型生物破乳剂产生菌Alcaligenes sp. S-XJ-1，分析菌体对谷氨酸和硝酸铵的利用过程，并建立菌体的生长及氮源利用模型。结果表明：氮源种类和投量是影响菌体产量和性能的重要因素；菌体对氮源的利用顺序依次为有机氮、氨态氮、硝态氮；谷氨酸作氮源时对生物破乳菌产量的促进效果最佳，相比于硝酸铵做氮源，该培养条件下，菌体最大比增长速率(µm)和产率系数(YP/S)更高，用于菌体代谢供能的氮源消耗量更低。

Abstract:

Six animo acids and ammonium nitrate were tested to study the effects of nitrogen sourceon the growth of cell-bound biodemulsifying strain of Alcaligenes sp. S-XJ-1. The utilization process of nitrogen source were studied when glutamic and ammonium nitrate was separately used as nitrogen source. The growth model and nitrogen utilization model of S-XJ-1 were built as well. The results showed that S-XJ-1 preferred to use organic nitrogen when organic nitrogen and ammonium nitrogen coexisted in the substrate, and ammonium nitrogen was preferred than nitrate nitrogen.The results also showed that the yield and property of biodemulsifying bacteria was significantly affected by the variety of nitrogen source,and glutamic was testified to be the best nitrogen source for S-XJ-1 among the six amino acids. When glutamic was used as nitrogen source, S-XJ-1 exhibited higher maximum specific microbial incremental rate(µm), higher utilization ratio of nitrogen source(YP/S) and lower energy cost.