枯草芽孢杆菌利用脂肪酸酯和氨基酸合成脂肽

Utilization of Fatty Acid Ester and Amino Acids by Bacillus Subtilis to Synthesize Lipopeptide

摘要:

利用脂肪酸酯为碳源培养枯草芽孢杆菌（B. subtilis）ATCC 6633合成脂肽的研究结果表明：6种食用油脂为碳源培养B. subtilis ATCC 6633均可合成脂肽.以食用油脂主要成分三油酸甘油酯为碳源时，谷氨酸作用下脂肽产量可从95 mg·L-1提高到183.5 mg·L-1.采用稳定性同位素技术发现菌体主要利用三油酸甘油酯水解产物甘油进行菌体和脂肽合成，谷氨酸的添加提高了脂肪酸酯利用率，促进碳源流向脂肽合成，谷氨酸则流向菌体合成，从而使生物量和脂肽产量提高.

Abstract:

The investigation mainly focused on the utilization of fatty acid ester by B. subtilis ATCC 6633 to synthesize lipopeptide. The results show that lipopeptide can be synthesized by using 6 kinds of edible oil as carbon source. When olein, the main composition of edible oil, was chosen as the carbon source , the yield of lipopeptide was enhanced from 95 mg·L-1 to 183.5 mg·L-1 under the action of glutamic acid (Glu). Through stable isotope probing, it was confirmed that glycerol, the hydrolysis product of olein, was of better use by the bacteria . With Glu addition, the utilization rate of fatty acid ester was improved and more carbon source was used to synthesize lipopeptide ,while Glu was mainly used to synthesize the bacteria, thus biomass and the yield of lipopeptide were enhanced.