外加氮源强化石油降解菌降解性能

Enhanced Biodegradation Characteristics of Oil Degrading Strains Supplemented with Nitrogen Source

摘要:

试验采用从天津、大连和上海附近海域筛选出的石油降解菌T4、R4和D3，研究了外加氮源对单一菌株和混合菌株降解柴油的影响.试验结果表明，外加氮源有效地促进了微生物对柴油的降解并增强了微生物细胞表面疏水性，R4和D3的最优氮源为NaNO3，T4的最优氮源为NH4NO3，最优碳氮比均为20:1；细胞表面疏水性与柴油降解率具有良好的相关性.分析微生物代谢产物发现其具有明显的脂肽结构且添加氮源后疏水基团含量明显增加.外加氮源可以使混合菌的柴油降解率大幅提高，其中复合菌T4/R4、T4/D3、T4/R4/D3表现出了高于理论值的协同降解作用.

Abstract:

The paper studies the effects of adding nitrogen source on single strains and mixed strains isolated from seawater of Tianjin, Dalian and Shanghai. The results show that adding nitrogen source promotes the biodegradation of diesel oil and enhances the cell surface hydrophobicity. NH4NO3 is the optimal nitrogen source to T4 while NaNO3 the optimal to D3 and R4 as well as the optimal ρ(C)/ρ(N) to the three strains is 20:1.There is a close relationship between biodegradation and cell surface hydrophobicity. It is found that there is a lipopeptide structure in the metabolin of petroleum degrading bacteria and the hydrophobic group increases significantly with the addition of nitrogen source. Adding nitrogen source improves biodegradation rate of microbial consortium greatly, in the meantime,T4/R4, T4/D3, T4/R4/D3 show the synergistic degradation.