The factors that affect the freezing process in supercooler was analyzed based on the classical nucleation theory in this paper. Then, the experiment bench which is used to make ice slurry by supercooled method were set up to research the freezing process in the supercooler. The impact mechanism of flow and heat transfer process to freezing in supercooler was explored preliminarily combining with the theory of boundary layer. The experimental results show that the supercooled water nucleation process is influenced mutally by the heat transfer surface tempeature and the disturbance of fluid. The nucleation is mainly affected by heat transfer wall temperature when the Re number is small or the temperature of secondary refrigerant is low and is mainly affected by the fluid disturbance when the Re number is large.