不同填料对蚯蚓生物滤池微生物酶活性的影响

Impacts of Filter Media on Enzymatic Activities in Earthworm Biofilter

摘要:

蚯蚓生物滤池是一种适用于分散式村镇污水污泥处理的生态型技术.填料是蚯蚓与微生物赖以生存的介质，对蚯蚓与微生物生理生态作用的发挥具有重要的影响.在可挥发性悬浮物质量浓度有机负荷为2.11 kg?m-3?d-1的条件下，考察木珠、瓷球和鲍尔环3种填料对蚯蚓生物滤池处理剩余污泥工艺性能的影响.通过蚯蚓酶活性和微生物酶活性的变化，分析3种填料作为蚯蚓生物滤池填料的可行性.结果表明,在3种填料的蚯蚓生物滤池均满足有机物(VSS)的减量率大于40%的前提下，与瓷球和鲍尔环相比，木珠蚯蚓生物滤池内，蚯蚓的超氧化物歧化酶（SOD）活性最低，酸性磷酸酶（ACP）活性最高，微生物的脱氢酶（INTDHA）活性最高.表明蚯蚓在木珠填料的微环境中受到的胁迫最小，且微生物降解能力最高，因此，木珠填料利于蚯蚓生物滤池工艺效能的发挥.

Abstract:

Vermifiltration is an ecological technology that is applied to decentralized wastewater and excess sludge treatment. Filter media significantly influence physiological and ecological functions of earthworms and microbes. At the organic loading (mass concentration of volatile suspended solids) of 2.11 kg?(m3?d)-1, the effects of the three filter media (wooden bead, porcelain ball and pall ring) on excess sludge treatment performance were investigated by exploring the variations of enzymatic activities of earthworms and of microbes. The results demonstrate that the VSS reduction by three earthworm biofilters can meet the requirement of execess sludge treatment (upwards of 40%). Notably, earthworm biofilters filled with wooden bead can offer the best habitat for earthworms and microbes. The SOD and ACP of earthworms have the lowest and highest values of the fiter media, respectively, indicating that earthworms suffer less adverse effect from wooded bead filter material. Furthermore, the highest DHA value of microbes also suggest that the wooden bead is the optimum filter material for biochemical degradtaion concideration. The earthworm enzymatic activities of superoxyde dismutase are the lowest and the acid phosphatase and the microbe dehydrogenase enzymatic activities are the highest. This indicates that and microbes perform best their degradation function. Therefore, wooden bead is good for performance of earthworm biofilters.