基于细胞培养的新型微流体加热器的开发与应用

Exploitation and Applied Study of a Novel Integrated Microfluidics heater for Cell Cutlure

摘要:

设计开发一种新型功能化的二维微流体加热器系统,用于哺乳动物细胞(Hela)的灌注培养及生长过程中的实时监控。该装置在无培养箱的条件下,可以为细胞提供生长所需的温度环境。采用聚二甲硅氧烷(PDMS)及多层控制阀门制作微流体通道,与采用镀有金和铬的玻璃片制作的加热片高度集成,构成一个加热片上具备九个加热单元的装置。通过阀门控制,装置各单元独立工作,控制各腔室细胞接种及排出。该系统可以为各腔室培养的细胞提供稳定及均匀的温度(37_oC),培养结果显示所培养细胞生长状况良好,表明该装置具有较好工作性能。

Abstract:

In this paper, we developed a functional and robust microfluidic perfusion-based 2-D cell culture system for mammalian cells (Hela) with long term monitoring. This device is capable of providing a closed environment for cell growth and manipulation without the need of an incubator. A 9-chamber poly(dimethylsiloxane) (PDMS) flow system with multi-layer Quake-valve control was merged with a microheater fabricated on an Aurum/Chromium (Au/Cr) wafer. By employing valve control and bypass, each chamber works independently, either feeding cells with desired density into the chamber or chasing cells out of it. The novel network microheater enable to provide a local temperature at 37°C with uniform temperature distribution for cells living in each chamber. Results show that cells growth characteristics and morphology in each chamber with different seeding density are comparable to those with conventional tissue cultural vessels.