A topology design method for the weld line location layout optimization of tailor welded blank(TWB) door is proposed in the paper. The multistiffness performance is required and the bidirectional evolutionary structural optimization (BESO) is considered as the basic design algorithm. Thus, the traditional interpolation model with Young’s module is changed into a novel model with thickness property. Finally, the sensitivity equations are derived from different stiffness requirements. The results show that the region boundary of different thicknesses is more obvious and the total weight of optimized door reduces by nearly 30% with only about 1% loss of its stiffness.