絮凝微生物的功能定位及其絮凝成分分析

Function Localization and Its Flocculating Ingredient Analysis of BioflocculantProducing Microorganism

摘要:

通过对絮凝微生物F2的质粒转化与质粒消除,对絮凝基因进行了初步定位, 分析了絮凝微生物的絮凝成分.利用高温SDS(十二烷基硫酸钠)法将F2质粒消除后仍具有絮凝特性,且质粒转化到无絮凝特性的大肠杆菌DH5α中,随着质粒的获得,这些转化子仍然无絮凝能力产生.说明F2的絮凝功能与质粒无关,初步证明絮凝基因不在质粒上,而在染色体DNA上.代谢成分受染色体DNA控制,与其相关的代谢途径为初级代谢.初级代谢循环为糖酵解代谢途径,产物为多糖类物质.

Abstract:

Preliminary localization of flocculating gene was conducted by processing plasmid transformation and plasmid elimination to bioflocculantproducing microorganism F2. An analysis of the relationship between the flocculating gene and metabolic pathway of flocculent bacterium shows F2 still has flocculent characteristic after plasmid elimination by high temperature SDS technique, and the bacillus coli DH5α without flocculent capability doesn’t obtain flocculent capability after acquiring the plasmid from F2.F2’s flocculent capability proves to be independent of its plasmid, which provides primary evidence that flocculent gene locates on chromosome DNA rather than plasmid. The metabolic components are controlled by chromosome DNA and the corresponding metabolic pathway is primary metabolism, which is characterized by taking glycolytic pathway as its cycle and saccharide substances as its metabolites.