The procedure of fatigue life prediction is illustrated.The multibody system(MBS) model of the rear axle and the suspension system of a car is built in the LMS.Virtuallab with an introduction of the virtual test rig concept.With the help of road test spectrum and the finite element analysis(FEA) method, the fatigue life of the rear axle is predicted successfully.The indoor test rig results validate the simuiation results.This method proves to be able to calculate the fatigue life of the components,which are under complicated forces.