Based on the principle of grid shear reinforcement, the concept of precast segmental girders with continuous longitudinal reinforcements across joints was proposed. Three test beams were designed, and the joint type was taken as the test parameter. The bending shear test was carried out with the research objects of crack development, reinforcement strain, failure mode, stiffness and bearing capacity. The test results show that the longitudinal reinforcement of webs across joints effectively bears the axial tension caused by shear force, and plays the same role of shear resistance as the stirrup. The continuous longitudinal reinforcement can restrain the development of oblique cracks and limit the width of main cracks, so as to effectively improve the stiffness of precast segmental beam structure. During the whole loading process from the cracking to the failure of continuous longitudinal reinforcement precast segmental beams, the structure near the joint satisfies the plane cross-section assumption, and the mechanical behavior is similar to that of the whole beam. The test results fully verify the necessity of continuous longitudinal reinforcement across the joint of precast segmental beams.