Research on the Improvement of the Stability of Solid Supported Bilayer Lipid Membranes

Abstract:

Solid supported bilayer lipid membranes (S-BLMs) was fabricated on the regenerated metal surface of stainless steel by using lecithin-decane solution as S-BLMs forming solution. The forming process of S-BLMs was researched by cyclic voltammetry, and the means to improve the stability of the S-BLMs were also investigated. The results indicate that the stability of the S-BLMs could be improved effectively by the introduction of some cholesterol into the lecithin-decane solution since it could make the arrangement of lecithin molecules more compact and order on the regenerated metal surface. On the other hand, the treatment of the regenerated surface of stainless steel could enlarge the diameter of the support which has the ability to form S-BLMs. In addition, the stability of S-BLMs could be further improved by the addition of cholesterol as well as the surface treatment of stainless steel.