Immobilization Methods of Electrodes for Toxic Microbial Biosensor

Abstract:

The bovine serum albumin-glutaraldehyde cross-linking process and polycarbonate membrane immobilization process were used to prepare E.coli microbial electrodes for toxic microbial biosensor, and their performance for heavy metals and toxic organic compounds toxicity determination were studied. The results showed that the sensitivity of the E.coli microbial electrodes prepared by GA-BSA immobilization method was very poor, and was not suit for toxicity assessment. E.coli microbial electrode prepared by polycarbonate membrane immobilization process exhibited high sensitivity and good stability for toxicity determination, and it could still suit for the toxicity assessment after 2 months storage at 4℃. The polycarbonate membrane immobilization process is very simple, and it can realize the reuse of electrodes and thus reduced the cost of toxicity determination with microbial biosensor.