Lysozymeliposome Synthesis and Its Capability in Biofilm Removal

Abstract:

Stable lysozymeliposomes were synthesized via reversephase evaporation.Lysozyme and its liposomes were separately used to remove the stable biofilms cultivated on stainless steel surface.The liposomes and biofilms were characterized by Zeta potential analyzer,transmission electron microscope(TEM),scanning electron microscope(SEM)and Fourier transform infrared(FTIR)spectrometer.The experimental results show that the liposomes are of the average diameter of 80~100 nm and an average entrapment efficiency of 82.4%.Under the same concentration condition,free lysozyme and lysozymeliposomes can effectively remove total biomass of the biofilm,which forms with a mixed bacterial consortium,up to 62.4 % and 86.5 %,respectively.Lysozymeliposome reduces bacterial in biofilm and solutions by 89.6% and 99.6%,respectively,in 24 hours.Therefore,lysozymeliposome can effectively control the biofilm pollution on the stainless steel surface.