Preparation and Drug Carrying Characteristics of Flurbiprofen Liposomes

Abstract:

Flurbiprofen liposomes were prepared by thin film evaporation and ultrasonic technique.The encapsulation efficiency and drug loading of flurbiprofen liposomes were determined with the protamine aggregation method.The impact factors on drug carrying characteristics of flurbiprofen liposomes were discussed.The results indicate that flurbiprofen liposomes have good dispersion and their average diameter are about 100-250 nm.Flurbiprofen molecules were located in the hydrophobic group region of liposomes and the partition coefficient KD of flurbiprofen between the liposomes and the aqueous phase was 815.6.The obtained liposomes have better entrapment efficiency and drug loading when the concentration of lecithin is 5.4×10-4 mol·L-1.With the increase of flurbiprofen/lecithin mass ratio,the drug loading of flurbiprofen liposomes increases,while its encapsulation efficiency decreases.Cholesterol can adjust the stability of liposomal membrane and the mass ratio of cholesterol to lecithin should be lower than 0.3.However,higher cholesterol concentration makes a great amount of cholesterol insert into membrane and leads to larger membrane rigidity,which decreases the entrapment efficiency and drug loading of liposomes.