Utilization of Fatty Acid Ester and Amino Acids by Bacillus Subtilis to Synthesize Lipopeptide

Abstract:

The investigation mainly focused on the utilization of fatty acid ester by B. subtilis ATCC 6633 to synthesize lipopeptide. The results show that lipopeptide can be synthesized by using 6 kinds of edible oil as carbon source. When olein, the main composition of edible oil, was chosen as the carbon source , the yield of lipopeptide was enhanced from 95 mg·L-1 to 183.5 mg·L-1 under the action of glutamic acid (Glu). Through stable isotope probing, it was confirmed that glycerol, the hydrolysis product of olein, was of better use by the bacteria . With Glu addition, the utilization rate of fatty acid ester was improved and more carbon source was used to synthesize lipopeptide ,while Glu was mainly used to synthesize the bacteria, thus biomass and the yield of lipopeptide were enhanced.