Preparation and Adsorption Properties of Laccase of Vesicle like Mesoporous Silica

Abstract:

Three dimensional hexagonal mesoporous silica (YPS, P means H3PO4) and vesicle like mesoporous silica (YNS, N means HNO3) were synthesized by the sol gel method using H3PO4 or HNO3 as catalyst, sodium coco glycerol (YCS) as surfactant, tetraethyl orthosilicate (TEOS) as silicon source. The as prepared YPS and YNS samples were calcined at different temperatures including 550 ℃, 700 ℃ and 850℃. The prepared samples were characterized by X ray diffraction (XRD), high resolution scanning electron microscope (HRSEM), high power transmission electron microscopy (HRTEM), nitrogen adsorption and micro electrophoresis, the effects of the calcination temperature on pore size, surface area were discussed. Moreover, the influences of the adsorption isotherm and the kinetics model of laccase on YPS and YNS samples of the isoelectric point, surface area, pore volume and other parameters were also discussed.

Key words:mesoporous silica; laccase; adsorption; structure