Modeling and Analysis of Thermal Resistance of the Base of a PCR Instrument

Abstract:

Based on the mathematics modeling of the polymerase chain reaction（PCR） instrument basement, the value of process thermal resistance of the temperature variation law was obtained. Then, the process thermal resistance value obtained was used for the calculation of the the similar temperature field of key parameters, and the temperature field of a complex model was also easily solved even with some dedicated parameters. The simulation result show a relationship between the thermal resistance and the characteristic of the base of the PCR instrument. An assumption was proposed as well as a mathematics description. Finally, the feasibility of the application of this assumption is proved by numerical computation.

Key words:polymerase chain reaction; temperature field; thermal resistance