Abstract:In this paper, we developed a functional and robust microfluidic perfusion-based 2-D cell culture system for mammalian cells (Hela) with long term monitoring. This device is capable of providing a closed environment for cell growth and manipulation without the need of an incubator. A 9-chamber poly(dimethylsiloxane) (PDMS) flow system with multi-layer Quake-valve control was merged with a microheater fabricated on an Aurum/Chromium (Au/Cr) wafer. By employing valve control and bypass, each chamber works independently, either feeding cells with desired density into the chamber or chasing cells out of it. The novel network microheater enable to provide a local temperature at 37°C with uniform temperature distribution for cells living in each chamber. Results show that cells growth characteristics and morphology in each chamber with different seeding density are comparable to those with conventional tissue cultural vessels.
