Function Localization and Its Flocculating Ingredient Analysis of BioflocculantProducing Microorganism

Abstract:

Preliminary localization of flocculating gene was conducted by processing plasmid transformation and plasmid elimination to bioflocculantproducing microorganism F2. An analysis of the relationship between the flocculating gene and metabolic pathway of flocculent bacterium shows F2 still has flocculent characteristic after plasmid elimination by high temperature SDS technique, and the bacillus coli DH5α without flocculent capability doesn’t obtain flocculent capability after acquiring the plasmid from F2.F2’s flocculent capability proves to be independent of its plasmid, which provides primary evidence that flocculent gene locates on chromosome DNA rather than plasmid. The metabolic components are controlled by chromosome DNA and the corresponding metabolic pathway is primary metabolism, which is characterized by taking glycolytic pathway as its cycle and saccharide substances as its metabolites.