Research on Improving Stability of Solid Supported Bilayer Lipid Membranes

Abstract:

Solid supported bilayer lipid membranes(S-BLMs)was fabricated on the regenerated metal surface of stainless steel by using lecithin-decane solution as S-BLMs forming solution. The forming process of S-BLMs was researched by cyclic voltammetry, and the means to improve the stability of the S-BLMs were also investigated. The results indicate that the stabilith of the S-BLMs can be improved effectively by the introduction of some cholesterol into the lecithin-decane solution since it helps the arrangement of lecithin molecules to be more compact and in order on the regenerated metal surface. On the other hand,the treatment of the regenerated surface of stainless steel can enlarge the diameter of the support which has the ability to form S-BLMs. In addition,the stability of S-BLMs can be further improved by both the addition of cholesterol and the surface treatment of stainless steel.

Key words:S-BLMs; lecithin; cyclic voltammetry; stability