Immobilization Methods of Electrodes for Toxicity Microbial Biosensor

Abstract:

The bovine serum albuminglutaraldehyde crosslinking process and polycarbonate membrane immobilization process were used to prepare E.coli microbial electrodes for toxicity microbial biosensor,and their performance for heavy metals and toxic organic compounds toxicity determination was studied.The results show that the sensitivity of the E.coli microbial electrodes prepared by GABSA immobilization method is very poor,and is not suitable for toxicity assessment.E.coli microbial electrode prepared by polycarbonate membrane immobilization process exhibits high sensitivity and good stability for toxicity determination,and is suitable for the toxicity assessment even after 2 months’ storage at 4 ℃.The polycarbonate membrane immobilization process is very simple,and the reusing of electrodes can be realized and thus the cost of toxicity determination with microbial biosensor reduces.